Annealing, Denaturation, Extension

You are correct that the annealing step is an important one in the PCR. In the annealing step, the temperature is lowered to 50-65°C to allow primers to bind to the single-stranded DNA. Which step allows the formation of single-stranded DNA? This needs to happen first before proceeding with the next two steps. Please think about this question and reconsider your response. 

Denaturation, Extension, Annealing
Denaturation, Annealing, Extension
Extension, Denaturation, Annealing

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
Node: 18562
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