Extension, Denaturation, Annealing

You are correct that all three steps are important for a PCR. In the extension step, the temperature is raised to around 72°C to allow the DNA polymerase to bind to single-stranded DNA to synthesize the new DNA strand by adding nucleotides to the primer. Which step allows the formation of single-stranded DNA? This needs to happen first before proceeding with the next two steps. Please think about this question and try again.

Denaturation, Extension, Annealing
Denaturation, Annealing, Extension
Annealing, Denaturation, Extension

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
Node: 18563
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