Ethanol

You consider the different reagents and reply that ethanol is the reagent that is responsible for denaturing proteins and helping in the breakdown of tissues and cells. Dr. Demetriou explains that this is incorrect and reminds you that ethanol is used to precipitate DNA and enhance its binding to the silica membrane in the spin column. He further explains that It helps to remove contaminants and facilitates the washing steps by making the DNA less soluble in the aqueous phase. Dr. Demetriou asks you to reconsider your answer.

Wash buffer
Proteinase K
Lysis buffers

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR) (987)
Node: 18521
Score:

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  • Ethanol

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