Proteinase K

You think very carefully about the different reagents so that you can identify the one that is responsible for denaturing proteins and helping in the breakdown of tissues and cells. You respond to Dr. Demetriou that proteinase K is the reagent in question. Dr. Demetriou reminds you that this enzyme digests proteins, including nucleases that could degrade DNA. He goes on to explain that it ensures that the DNA is released from protein-DNA complexes and remains intact during the purification process. Finally, he adds that proteinase K may also help to lyse cells and tissues, however another reagent used is more important in this respect. You thank Dr. Demetriou for this helpful response and proceed to reconsider your answer.