Start by selecting the pipette appropriate for your volume.

Dr. Smith: ‘This is correct!’ She proceeds to demonstrate the correct procedure.

1. Select the Appropriate Pipette: Choose a pipette that matches the volume range required for your task.

2. Attach a Pipette Tip: Firmly attach an appropriate, sterile tip to the pipette. Ensure it fits snugly to avoid leaks.

3. Set the Desired Volume: Adjust the pipette to the required volume using the volume adjustment dial. Ensure it locks in place.

4. Aspiration:

  • Press the plunger to the first stop (the initial resistance point).

  • Immerse the tip vertically just below the surface of the liquid to be aspirated.

  • Release the plunger slowly and steadily to aspirate the desired liquid volume.

5. Dispensing:

  • Align the tip with the receiving vessel and press the plunger to the first stop to dispense the liquid.
  • Press the plunger to the second stop to expel any remaining liquid in the tip.

6. Dispose of the Tip: Eject the used tip into a waste container using the tip ejector button, being careful not to touch the tip with your hands.

7. Repeat as Necessary: If more repetitions are needed, attach a new tip and repeat the process.

For a more extensive video visit this link: How to pipette correctly – a short step-by-step introduction into proper pipetting (https://www.youtube.com/watch?v=QGX490kuKjg) 

 

Dr. Smith instructs the students to pipette 1000 μL and watches Emma's careful attempt noting her frustration with the small droplets lingering in the tip.

 

What is the most likely error? 

Option 1: Emma is likely releasing the plunger too quickly during aspiration, which can cause air bubbles to form in the tip and lead to inconsistent volume uptake (e.g 1050 μL).

Option 2: Emma is likely submerging the pipette tip too deeply, causing liquid to be drawn into the tip due to increased pressure differences or capillary action. 

Option 3: Emma is holding the pipette at an incorrect angle (rather than vertically) while aspirating or dispensing can lead to incorrect volume measurements.

  • Option 2
  • Option 3
  • Option 1

Map: CS12 - USING BASIC LABORATORY EQUIPMENT (1062)
Node: 20038
Score:

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  • E nevoie de o mărire mai mare pentru a observa celulele
  • (1) prometafase, (2) interfase, (3) telofase, (4) prometafase, (5) metafase y (6) anafase
  • Ask him to lie on his right side with his knees drawn up towards his chest
  • Taq polymerase
  • (1) profase, (2) telofase, (3) telofase, (4) prometafase, (5) metafase y (6) anafase
  • Νεφελοποιητής Σαλβουταμόλης
  • Ενσωματωμένες πρωτεΐνες
  • Pine tree cones
  • Supracolorarea lamei
  • Answer 3 - hypotonic is 6% NaCl, hypertonic is 0.9% NaCl, isotonic is distilled water
  • Setting up the reaction
  • To ensure proper folding and function of the restriction endonuclease, maximizing its catalytic activity
  • Proceed to thorax CT.
  • Hair
  • Ignorar la probabilidad porque ya sabemos que la FQ es recesiva
  • (1) profase, (2) interfase, (3) telofase, (4) prometafase, (5) metafase y (6) anafase
  • They will buy a bed.
  • Οξεία νεφρική και γαστρεντερική βλάβη λόγω υπερβολικής χρήσης ΜΣΑΦ ή/και ακεταμινοφαίνης
  • Root Node
  • (1) prophase, (2) interphase, (3) telophase, (4) prometaphase, (5) metaphase and (6) anaphase
  • Place the diaphragm of your stethoscope over the branchial artery
  • Amplificarea ADN-ului
  • TURB
  • O canapea și o masă.
  • Anamnestic examination
  • Microscopic Observation
  • Cystoscopic Exam
  • Start iv UFH adjusted for body weight.
  • De la 5nm până la 100μm
  • Node 9: Reassure her receive continuous supportw node
  • (1) profază, (2) telofază, (3) telofază, (4) prometafază, (5) metafază and (6) anafază
  • (1) prometafază, (2) interfază, (3) telofază, (4) prometafază, (5) metafază and (6) anafază
  • Gel electrophoresis
  • DNA Amplification
  • DNA Ligase
  • Ακτινογραφία Θώρακος
  • Συνταγογραφείτε ΜΣΑΦ και/ή ακεταμινοφαίνη για τη διαχείριση του πόνου του
  • Hear the first Korotkov sound
  • Option 1
  • He thinks that she is not as nice as Mrs Mary was.
  • OSCE Station 4 - Cardiovascular auscultation
  • Opción 2: Sólo B es correcta
  • Τρόπος ζωής του ασθενούς
  • Traits
  • Υποτασική αιμόσταση
  • He accepts it.
  • Observar la sección equivocada del extremo
  • The patient has diabetes.
  • Χορήγηση σαλβουταμόλης
  • He is mad and doesn’t speak to her.
  • Option 2
  • Description 1
  • A microscope that uses electron source to magnify objects
  • Taq polymerase
  • Start by immersing the pipette in the liquid you intent to aspirate.
  • Description 3
  • ΑΙΜΟΛΗΨΙΑ
  • Un microscop care folosește o sursă de lumină pentru a mări obiectele
  • Start by immersing the pipette in the liquid you intent to aspirate.
  • On the left edge of the sternum in the 2nd intercostal space
  • Larger area sample and when surface information is needed
  • ΚΛΙΝΙΚΗ ΕΞΕΤΑΣΗ
  • HeLa cells are naturally resistant to all drugs.
  • Diploid and Haploid
  • Prácticas de laboratorio criminalístico
  • Node 12: Move to a discussion on troubleshooting
  • Start
  • Un microscop care folosește o sursă de fluorescență pentru a mări obiectele
  • Restriction Endonuclease Digestion: Optimal Conditions
  • Pipettes
  • Node 14: Provide a support contact for technical issues
  • Start by selecting the pipette appropriate for your volume.

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