Optimal pH and temperature for amylase

James: I understand now. We definitely need to consider the optimal conditions for an enzyme, which might vary for different enzymes. How can we figure out the optimal pH and temperature for an enzyme? How can we test the effect of pH and temperature on an enzyme?’

Prof. Wilson: This is the lab practical for today! We will use the enzyme amylase. Do you remember the substrate and product for this important enzyme?’