Low-glucose media for energy-limited systems (DMEM-LG)

Elena: ‘How about a low-glucose medium? Maybe HeLa cells don’t always need that much energy.’

 

 

LabMal: https://labmal.com/product/dmem-with-low-glucose-without-l-glutamine-500ml/ 

 

Dr. Lee: ‘Not quite, Elena. Low-glucose DMEM, which contains only 1 g/L of glucose, is often used for studying energy-limiting conditions or slower-growing cell lines. However, HeLa cells are fast-growing and require more energy to maintain their rapid division. If we start them on a low-glucose medium, we might unintentionally create stress conditions before even introducing the experimental variable.’

Dr. Lee: ‘Have a look at the content table and let’s think again about the best media’

 

Table 1: Composition of LB Broth, DMEM, and DMEM-LG (Low-Glucose)

Component

LB Broth

DMEM (High-Glucose)

DMEM-LG (Low-Glucose)

Glucose

Not included

4.5 g/L

1.0 g/L

Amino Acids

Not included

Contains essential and non-essential amino acids (e.g., glutamine, glycine, alanine)

Same as DMEM (High-Glucose)

Vitamins

Not included

Includes essential vitamins (e.g., folic acid, riboflavin, pyridoxine)

Same as DMEM (High-Glucose)

Salts

Sodium chloride (5 g/L)

Balanced salts (e.g., sodium chloride, potassium chloride, magnesium sulfate)

Balanced salts

pH Buffer

None

Sodium bicarbonate

Sodium bicarbonate

 

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