Setting up the reactionDr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific. https://www.thermofisher.com/order/catalog/product/FD0275
You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.
You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. Which technique is appropriate to visualize the results? |
Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA_1 (1022)
|
||||||||||||
Review your pathway |