pH 10.0

Prof. Wilson: Let's find out based on your results.

                          Results: Optimal pH for amylase activity

James: Oh.. at pH 10 the mixture remained dark blue even at the end of the experiment at 10 minutes. This means maltose has not been produced and the rate of this reaction is super slow, Prof. This cannot be the optimal pH for amylase as the enzyme activity is low.

Prof. Wilson: Correct! Let’s try again!

What is the correct answer?