DNA template, Primers, DNA polymerase, rNTPs, Buffer solution

That’s right, the DNA template, i.e. the DNA you have already isolated, is important since it contains the target sequence to be amplified. Thereafter, you also need primers, which are short sequences of nucleotides that provide the starting point for DNA synthesis, in this case they have been synthesized to bind to the transgene in the mouse model, which is not found in control mice. You are correct that you also need a DNA polymerase enzyme to synthesize the new DNA strands and you need the building blocks for the synthesis of the new DNA strand. The buffer solution is important to maintain the optimal pH and ionic strength for the activity of the polymerase. You need to consider though if rNTPs (ribonucleotide triphosphates) are the appropriate nucleotides. Remember that they are the building blocks for RNA, not DNA, synthesis. Please think about this and try again.