DNA Isolation: Experimental steps

You decide to also watch a video of the procedure before you do it yourself.

 

You wean eight mice at 21 days post-birth, tail-clip and ear-mark them appropriately. 

 

Image website: https://www.rvc.ac.uk/research/research-centres-and-facilities/rvc-animal-welfare-science-and-ethics/news/identical-mice-which-is-the-kindest-way-to-mark-them

You proceed to isolate DNA from the tails, using the DNA purification kit (QIAGEN), according to the manufacturer’s instructions, as shown below.

Image from: https://www.protocols.io/view/dna-extraction-from-filters-using-qiagen-dneasy-an-j8nlk61wv5r7/v1?step=9&preview_img=8D584F46344311EEAFD10A58A9FEAC02

  • Genotyping mice for the noveltin gene I: PCR Reagents

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR) (987)
Node: 18525
Score:

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  • Jeg bestemmer å ta det opp med henne så fort så mulig
  • Insulin
  • Surfactant -Frusemide
  • Refer to gastroenterologist
  • Commentary
  • Pedro y su padre juegan juntos.
  • new node
  • Steroids -Antibiotics
  • Râde de mama lui.
  • Meet the patient
  • Έναρξη
  • Exlpain the procedure and ask for consensus
  • Jeg forklarer foreldrene at jeg tar opp saken med assistenten så fort så mulig
  • Case
  • Learning Point
  • Antibiotics-Echocardiogram
  • Antibiotics-Antibiotics
  • Surfactant -Antibiotics
  • AISLAMIENTO DE ADN Y REACCIÓN EN CADENA DE LA POLIMERASA (PCR)
  • Επιφανειοδραστικός παράγοντας - Φουροσεμίδη
  • Pedro y su padre dialogan y pasan tiempo juntos.
  • Give Intravenous Antibiotics
  • El își ignoră mama.
  • Propranolol
  • Steroids-Echocardiogram
  • IDENTIFICATION OF BIOMOLECULES
  • Second
  • Jeg henviser foreldrene til styren. Det er faktisk styren sin jobb å ha oversikt over personalets sitt henvender til barn
  • IZOLAREA AND-ULUI ȘI REACȚIA ÎN LANȚ A POLIMERAZEI (PCR)
  • Αντιβιοτικά - Φουροσεμίδη
  • Refer for endoscopy for biopsy
  • Mrs. Rahma's Knee Dilemma
  • Χορηγείτε ενδοφλέβια αντιβιοτικά
  • Pedro y su padre discuten.
  • Start gluten free diet
  • Last
  • Επόμενο
  • IDENTIFICATION OF BIOMOLECULES
  • Ethanol
  • Refer to gastroenterologist
  • DNA Isolation: Experimental steps

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