Identification of mice carrying the transgene.

You isolate DNA again and remember to store it at 4oC. You visualize the results on an agarose gel once you re-run the PCR reaction, as shown below. 

You need to interpret the results and decide which mice are non-transgenics so that they can be sacrificed. 

Which mouse or mice DO NOT carry the noveltin transgene?


  • 4
  • 2, 4, 6
  • 2
  • 2, 4

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
Node: 18571
Score:

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Review your pathway

  • Experiment #3: Assessing Seed Germination Rates
  • Η φυγόκεντρος – διαδικασία και ασφάλεια
  • Experiment #2: Isolating Blood Cells from Plasma
  • Experiment #4: Cell Fractionation and Separation
  • Experiment #1: Monitoring Algal Growth in Water Samples
  • La placa no se incubó a la temperatura o al nivel de CO₂ adecuados
  • Further Reflection
  • The quality of the DNA sample is poor.
  • A fire extinguisher is used when there is a fire in the lab. We use the fire blanket to put out the fire on a person (STOP, DROP, ROLL)
  • Diluție 1/10000
  • Bacterial load
  • Diluție 1/1000
  • The centrifuge – process and safety
  • Diluție 1/10
  • Final Summary
  • Identification of mice carrying the transgene.

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