Denaturation, Annealing, Extension

You are absolutely right! 

The first step is denaturation, which allows the double-stranded DNA to separate into two single strands by heating it to around 94-98°C. In the next step of annealing, the temperature is lowered to 50-65°C to allow primers to bind to the single-stranded DNA. Finally, the extension step is carried out by raising the temperature to around 72°C to allow the DNA polymerase to synthesize the new DNA strand by adding nucleotides to the primer.

  • PCR Experiment

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
Node: 18561
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  • Node 19: Sharing Success Stories
  • Observarea la microscop
  • The balance may not have been on a level surface, causing inaccurate readings.
  • Pătratul Punnett 1
  • Παρατήρηση στο μικροσκόπιο (φυτικό κύτταρο)
  • Experimental Setup #2
  • Laboratory Scales II
  • Tehnica Western Blotting
  • Carbo-Platinum + Gemcitabine
  • Results
  • Montaje experimental #3
  • Eye wash
  • Sunt necesare mostre mici și informații structurale interne
  • pH 8.0
  • Images from TEM and SEM
  • Quantification of bacterial load: Serial dilutions
  • The multi-child scenario.
  • You and Emma might have forgotten to tare the balance with the plastic bag in place.
  • Electroforeză în gel
  • Recommend strong antiseptic without guidance
  • pH 7.0
  • pH 3.0
  • Focus only on glucose control
  • pH 7.0
  • Dacă vrem să ne spălăm ochii pentru că s-au uscat
  • Getting better?
  • The reason
  • pH 8.0
  • Cuantificarea încărcării bacteriene: Diluții în serie
  • Denaturation, Annealing, Extension

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