Genotyping mice for the noveltin gene I: PCR Reagents

You are very excited to set up your first PCR experiment. You remember that you had a Biology lab as a Year 1 student where you learnt about the rationale of this experimental technique and recall that PCR can amplify specific segments of DNA. This is important in your experiment, considering the low concentration of DNA isolated from mouse tails. You decide to review the reagents that are used in a PCR experiment. 

Which of the following lists includes all the essential reagents required to run a PCR?

  • DNA template, RNA polymerase, dNTPs, Buffer solution
  • DNA template, Primers, RNA polymerase, dNTPs, Buffer solution
  • DNA template, Primers, DNA polymerase, rNTPs, Buffer solution
  • DNA template, Primers, DNA polymerase, dNTPs, Buffer solution

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
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