Annealing, Denaturation, Extension

You are correct that the annealing step is an important one in the PCR. In the annealing step, the temperature is lowered to 50-65°C to allow primers to bind to the single-stranded DNA. Which step allows the formation of single-stranded DNA? This needs to happen first before proceeding with the next two steps. Please think about this question and reconsider your response. 

  • Denaturation, Extension, Annealing
  • Denaturation, Annealing, Extension
  • Extension, Denaturation, Annealing

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR) (987)
Node: 18533
Score:

reset

OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Para evitar que se rompa o se ensucie fuera del laboratorio
  • Προσδιορισμός γονότυπου ποντικιών για το γονίδιο της νοβελτίνης II: Διαδικασία PCR
  • Η ποδιά εργαστηρίου μπορεί να μεταφέρει επικίνδυνα υλικά στον εξωτερικό χώρο και να μολύνει την περιοχή
  • Desnaturalización, Alineación, Extensión
  • Desnaturalización, Extensión, Alineación
  • Epilogue
  • Για να αποφύγουμε να μας μαλώσει ο καθηγητής μας
  • Description 1
  • Se roagă.
  • Live specimens
  • Extension, Denaturation, Annealing
  • Answer 3
  • O pai dele está a tentar cozinhar.
  • Plasarea celulelor pe lamele de cultură
  • Denaturation, Annealing, Extension
  • Εργαστηριακοί Ζυγοί I
  • Extensión, Desnaturalización, Alineación
  • Denaturation, Extension, Annealing
  • Which of the following values are detected?
  • Annealing, Denaturation, Extension

Reminder

empty_reminder_msg

FINISH

Time is up