DNA Isolation: Experimental steps

You decide to also watch a video of the procedure before you do it yourself.

 

You wean eight mice at 21 days post-birth, tail-clip and ear-mark them appropriately. 

 

Image website: https://www.rvc.ac.uk/research/research-centres-and-facilities/rvc-animal-welfare-science-and-ethics/news/identical-mice-which-is-the-kindest-way-to-mark-them

You proceed to isolate DNA from the tails, using the DNA purification kit (QIAGEN), according to the manufacturer’s instructions, as shown below.

Image from: https://www.protocols.io/view/dna-extraction-from-filters-using-qiagen-dneasy-an-j8nlk61wv5r7/v1?step=9&preview_img=8D584F46344311EEAFD10A58A9FEAC02

Genotyping mice for the noveltin gene I: PCR Reagents

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR) (987)
Node: 18525
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