Experimental set-up

Dr. Lee instructs the students to draw a diagram of the 24-well plate and incorporate two concentrations of Supplement X. He draws an empty 24-well plate and asks you to copy and label. 

C:\Users\nicolaou.s\Dropbox\1. RESEARCH PROJECTS\EDUCATION\1. Strategic partnership\0. March 2023\WPs\WP3\Case studies\Case Study (CS14) - Cell culture\24 well plate.jpg

24-well plate

Elena: ‘Dr. Lee, why only two concentrations of Supplement X?’

Dr. Lee: ‘Good question, Elena. Two concentrations let us observe a basic dose-response relationship without overcomplicating the setup. If we see significant differences between the low (10 μL) and high doses (50μL), it’s a great starting point for refining future experiments with additional concentrations.’

What should be included in your experimental set-up?

 

1. Experimental Setup #1: Triplicates of (a) cells with media only, (b) cells with a low dose of Supplement X and (c) cells with a high dose of Supplement X NODE 14

2. Experimental Setup #2: Triplicates of (a) cells with media only, (b) cells with a low dose of Supplement X, (c) cells with a high dose of Supplement X and (d) media only NODE 15

3. Experimental Setup #3: Triplicates of (a) media only, (b) cells with a low dose of Supplement X, (c) cells with a high dose of Supplement X, (d) cells with media only and (e) cells with stress media and Supplement X NODE 16

  • Experimental Setup #3
  • Experimental Setup #1
  • Experimental Setup #2

Map: CS14 - CELL CULTURE_3 (1067)
Node: 20161
Score:

reset

OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Genotipo y Fenotipo
  • Dominante y recesivo
  • De la albastru la violet
  • Start a therapy
  • De la albastru la roșu
  • His father is trying to cook.
  • Epilog
  • El preferă să stea pe canapea și să se relaxeze.
  • LABS
  • Pentru a stabiliza structura dublei helixuri a ADN-ului, asigurând o scindare precisă
  • Digestión del ADN con endonucleasas de restricción: Selección de la enzima adecuada
  • Digestia cu endonucleaze de restricție: Condiții optime
  • Λάβετε επιπλέον ιστορικό
  • Pregătirea lamei metoda 3
  • EMB
  • ECG
  • Node 3: Address each concern in detail
  • Todas las áreas analizadas están contaminadas
  • Las áreas B, C y D están contaminadas
  • Chest x-rays
  • Taq polimerază
  • Διώχνετε την εγγονή της κυρίας Σοφίας
  • MAC
  • LB
  • 37oC, 1% O2 levels, 5% CO2 levels.
  • Experiment #2: Isolating Blood Cells from Plasma
  • Experiment #4: Cell Fractionation and Separation
  • Profază, prometafază, metafază, anafază, telofază
  • Vârful rădăcinii de ceapă
  • 37oC, atmospheric O2 levels, atmospheric CO2 levels.
  • Answer 3
  • Start
  • Experimental set-up

Reminder

FINISH

Time is up