37⁰C, atmospheric O2 levels, 5% CO2 levels.

Elena: ‘I think this might be the best option. It includes CO₂, which I remember you said is vital for buffering the media, and DMEM is the appropriate media with glucose and serum.’

Dr. Lee: ‘That’s correct, Elena. Mammalian cells like HeLa require CO₂ to maintain pH balance with bicarbonate-buffered media, and DMEM with glucose and serum supports their metabolic and growth needs. These conditions mimic those in the human body, making them ideal for maintaining regular cell physiology. Proceed to plate your cells.’ 

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