Start by immersing the pipette in the liquid you intent to aspirate.

Dr. Smith: ‘I see that you are eager to start, but let's pause for a moment. When working with pipettes, it's crucial to first consider the volume of liquid you intend to handle. Selecting the appropriate pipette based on volume ensures accuracy and precision, which are vital in any experimental setup."

She directs everyone to their notes. "Have a look at the table provided in your materials. It outlines different pipettes and their typical applications—everything from minute microliter volumes up to larger milliliter quantities. Understanding this allows you to match the pipette to your task effectively."



Pipette Type

Volume Range (µL)

Typical Use Cases

P2 or P10

0.1 - 2 µL or 0.5 - 10 µL

Microliter volumes, DNA assays, PCR setup

P20

2 - 20 µL

Small volume transfers, molecular biology

P100

10 - 100 µL

General-purpose transfers, sample aliquots

P200

20 - 200 µL

Moderate volume transfers, standard assays

P1000

100 - 1000 µL

Larger volume transfers, reagent preparation

P5000 or P10mL

1 - 5 mL or 1 - 10 mL

Bulk transfers, solution preparation

 

With a supportive smile, she continues, "Choosing the right tool is as fundamental as how you employ it. Proper selection helps prevent errors and maximizes your experiment's reliability.’

 

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