Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Microscopic Observation
  • Spectrophotometer
  • Gel electrophoresis
  • Western Blot

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA_ES (1021)
Node: 19394
Score:

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  • Western Blot
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  • 1) prophase, (2) telophase, (3) telophase, (4) prometaphase, (5) metaphase and (6) anaphase
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  • Configurația experimentală #2
  • Setting up the reaction

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