Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

Western Blot
Spectrophotometer
Microscopic Observation
Gel electrophoresis

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA_ES (1021)
Node: 19394
Score:

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OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Ramipril
  • Opțiunea 1: Pentru siguranța noastră și a colegilor noștri în timpul și după practica de laborator
  • Opțiunea 2: Pentru a identifica și manipula corespunzător materialele periculoase din laborator
  • Καλείτε τους χειρουργούς
  • Έκβαση περιστατικού
  • Digestia ADN-ului cu endonuclează de restricție: Selectarea enzimei potrivite
  • Lansoprazole
  • Στο θάλαμο
  • Start B12 administration
  • Metolazone
  • Social History
  • Bisoprolol
  • Pogovorila se bosta o odhodu na počitnice.
  • Tipos de microscopio electrónico
  • Αρχίζει να γυμνάζεται με επαγγελματία γυμναστή
  • The result
  • Folosește pătratele Punnett și regula înmulțirii
  • The result
  • Jure bo mamo prosil za denar.
  • Selitev v nov dom.
  • Καλείτε την ομάδα της Μονάδας Εντατικής Θεραπείας
  • High-glucose media for fast-growing cells (DMEM)
  • Plating your cells
  • History of Medication Compliance
  • Interphase, prophase, prometaphase, metaphase, anaphase, telophase
  • The result
  • Amoxicillin
  • The result
  • Κουράζεται και επιστρέφει στη λήψη αγχολυτικών φαρμάκων
  • Media enriched for bacterial growth (LB Broth)
  • Start plasma exchange (PEx) and steroids
  • Concentrare pe cariotipuri
  • Fehling’s Test Procedure
  • Low-glucose media for energy-limited systems (DMEM-LG)
  • Prophase, prometaphase, metaphase, anaphase, telophase
  • Ignorați probabilitatea deoarece știm deja că FC este recesiv
  • To stabilize the DNA double helix structure, ensuring precise cleavage
  • Workup for potential underlying causes
  • To enhance the binding affinity of restriction endonucleases to non-specific sequences
  • Έκβαση περιστατικού
  • To generate single stands of DNA, ensuring efficient cleavage
  • To ensure proper folding and function of the restriction endonuclease, maximizing its catalytic activity
  • Setting up the reaction

Reminder

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FINISH

Time is up