Taq polymerase

Eric: How about Taq polymerase? Would that work for our DNA digestion?

You: Taq polymerase makes me think of PCR. Dr. Greene, could you clarify whether this enzyme is suitable for digesting DNA?

Dr. Greene: Absolutely. Taq polymerase is a thermostable enzyme used to synthesize DNA during PCR. It's responsible for amplifying DNA by adding nucleotides to the 3' end of a DNA strand during repeated thermal cycles.

Eric: So, it's not involved in cutting DNA, is it?

Dr. Greene: Precisely. Taq polymerase is crucial for replicating DNA rather than digesting it into fragments. For our purposes, where we need specific DNA cuts for RFLP, Taq polymerase wouldn't be the right choice.

You: That makes sense. We need an enzyme that cleaves DNA, like a restriction enzyme, to perform the actual cutting and fragmenting of the DNA.

Dr. Greene: Exactly. Should we give it another try?