Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Microscopic Observation
  • Western Blot
  • Gel electrophoresis
  • Spectrophotometer

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
Node: 19338
Score:

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OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Structure the flow
  • Delay wound assessment until an in-person visit
  • Τηλεφώνησες στους φίλους σου και δέχτηκαν με χαρά να βοηθήσουν.
  • Τα ούρα του ασθενούς δεν περιέχουν γλυκόζη
  • Celecoxib
  • Opțiunea 2
  • Learn about results in healthy adults
  • Descrierea 3
  • Επίσκεψη για επανέλεγχο στους τρεις μήνες
  • Social History
  • Περιγραφή 3
  • His nearest neighbour
  • Labetalol (third generation beta-blocker)
  • Τεστ Biuret
  • Ask the patient to walk or change position (risk of falling).
  • Centrarse en los cariotipos
  • Εστίαση στους Καρυότυπους
  • Start a Treatment
  • Jonah prefere ficar sozinho.
  • MAC
  • Balanzas de laboratorio II
  • Results from liquid cultures.
  • Verapamil
  • Glomerular filtration rate
  • A higher magnification is needed to observe the cells
  • Vitamin K
  • Your detail explanation
  • Option 3
  • Spironolactone
  • Option 2
  • Spironolactone (aldosterone/mineralocorticoid receptor antagonist)
  • State that assessment cannot continue without a clear image
  • Ευχετήριες κάρτες.
  • Ask the patient to take a clear photo of the wound (10–15 cm distance, adequate lighting) and upload it to the app
  • Bisoprolol (beta blocker)
  • Respond bluntly: “That is common in diabetes.”
  • Τεστ αναγνώρισης πρωτεϊνών
  • Setting up the reaction

Reminder

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FINISH

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