Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Spectrophotometer
  • Microscopic Observation
  • Gel electrophoresis
  • Western Blot

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
Node: 19338
Score:

reset

OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Quantification of bacterial load: Inoculating the bacterial samples on agar plates
  • Blame the patient for poor network connection
  • Menopausal Flushing
  • Arthritis
  • No
  • The spectrophotometer (The centrifuge)
  • Osteoporosis
  • Recommend strong antiseptic without guidance
  • Enfortumab-Vedotin
  • 1/100
  • Allow everyone to speak freely
  • Delay education until an in-person visit.
  • 1/10
  • Use visual icons
  • Romosuzumab
  • Forbid herbal remedies without explaining scientific risks or interactions
  • Focus only on glucose control
  • Βακτηριακό φορτίο
  • Provide tele-wound care education
  • Epilogue
  • Explain medications quickly without checking understanding
  • Setting up the reaction

Reminder

empty_reminder_msg

FINISH

Time is up