Reverse transcriptase

Eric: Let's consider using reverse transcriptase. Could that be suitable for our DNA digestion process?

You: I remember reverse transcriptase from our studies on RNA. Dr. Greene, could you give us a deeper insight into whether this enzyme would be appropriate?

Dr. Greene: Sure! Reverse transcriptase is an enzyme that plays a key role in converting RNA into complementary DNA (cDNA). It's widely used in molecular biology when working with RNA templates, particularly in generating cDNA libraries or amplifying RNA sequences using reverse transcription PCR.

Eric: So its main function is related to working with RNA, not directly cutting DNA?

Dr. Greene: Exactly! Reverse transcriptase doesn't have the ability to cleave or cut DNA. It's crucial for processes involving the conversion of RNA to DNA but not for generating the specific DNA fragments needed in our RFLP analysis for forensic purposes.

You: Got it, Dr. Greene. 

Dr. Greene: Let’s give it another try!