Benedict test

Dr Brown explains that the Benedict test is another test that indicates the presence of reducing sugars. 

Benedict’s reagent is a bright royal blue color. It is made up of a solution of copper sulfate, sodium carbonate and sodium citrate. In the presence of a reducing sugar, it accepts electrons causing the color change. The solution also turns cloudy due to the formation of a precipitate of cuprous oxide. Any color change indicates the presence of a reducing sugar, the degree of color change depends on the concentration of the reducing sugar. A yellow or green color indicate a low concentration of reducing sugar, while a red or orange color are indicative of a large concentration of reducing sugar. 

It is carried out similarly to Fehling’s test in that 2mL of Benedict’s reagent is added to 2 mL of the sample solution in a test tube. The mixture is then heated in a boiling water bath for 2 minutes and color changes are observed. The image indicates a negative test, which remains blue (left tube) and three positive (right three tubes).

Website:https://bio.libretexts.org/Courses/Harrisburg_Area_Community_College/BIOL_101%3A_General_Biology_l_-_Laboratory_Manual/01%3A_Labs/1.03%3A_Organic_Molecules 

Dr. Brown asks you to re-consider your answer. Considering that you are a visual learning, you also watch a video to gain a better understanding of this test.

  • Xanthoproteic test
  • Diphenylamine test
  • Biuret Test

Map: CS5 - BIOMOLECULES_ES (1002)
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