Start (Sample Collection)

You and your lab partners, Sarah and Emily, will visit a local pond to collect water samples. The instructor, Professor Johnson, explains that you will be examining the diverse microbial life present in the pond water samples collected from a local ecosystem. The goal is to observe and identify different microorganisms such as algae, protozoa, and bacteria, as well as any other microscopic organisms that may be present.

After 2 hours you have collected the samples and now you need to decide the best microscopic technique to help you study the microorganisms you collected. 

Prof. Johnson: "Students, when deciding on your observation parameters, keep these guidelines in mind: 

1. Size of the organism: Take note of the organism's size to ensure it fits within the field of view at the chosen magnification.

2. Magnification: Select a magnification level that provides adequate detail without compromising the clarity of the image.

3. Resolution: Prioritize selecting a magnification that offers sufficient resolution to distinguish fine structures within the specimens. 

4. Ability to observe living specimens: If observing living specimens, choose a method that minimizes disruption to their natural state.

Consider these factors carefully to make the most appropriate choice for your observations."