DNA Isolation: Experimental steps

You decide to also watch a video of the procedure before you do it yourself.

 

You wean eight mice at 21 days post-birth, tail-clip and ear-mark them appropriately. 

 

Image website: https://www.rvc.ac.uk/research/research-centres-and-facilities/rvc-animal-welfare-science-and-ethics/news/identical-mice-which-is-the-kindest-way-to-mark-them

You proceed to isolate DNA from the tails, using the DNA purification kit (QIAGEN), according to the manufacturer’s instructions, as shown below.

Image from: https://www.protocols.io/view/dna-extraction-from-filters-using-qiagen-dneasy-an-j8nlk61wv5r7/v1?step=9&preview_img=8D584F46344311EEAFD10A58A9FEAC02

Genotyping mice for the noveltin gene I: PCR Reagents

Map: CS4 - ISOLATION OF DNA AND POLYMERASE CHAIN REACTION (PCR)_SPANISH (988)
Node: 18554
Score:

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  • Testul emulsiei
  • Testul Fehling
  • Opțiunea 1
  • Option 3
  • They will buy furniture.
  • Ethanol
  • Follow up visit with patient after a month
  • Θα χορηγήσεις φαινοβαρβιτάλη;
  • Testul Sudan III
  • Wash buffer
  • Option 2
  • Opțiunea 2
  • Labs
  • Ζητάτε εργαστηριακές εξετάσεις, ακτινογραφίες και εξηγείτε περισσότερα για τη διαχείριση του πόνου.
  • Μοριακή κλωνοποίηση
  • Επιλογή 2
  • Assess the situation to identify any potential risks to yourself or the victim. Secure the victim with the help of a third party.
  • Option 1
  • Iodine Test
  • Node 2: Discuss Maria's feelings and concerns
  • Family
  • Clinical examination
  • Environmental Exposure
  • Opțiunea 3
  • Συνεχίζει τη Γνωσιακή - Συμπεριφορική θεραπεία
  • Opción 3
  • Opción 2
  • Lysis buffers
  • Remote Pathological anamnesis
  • Opción 1
  • Proteinase K
  • Un microscop care folosește o sursă de electroni pentru a mări obiectele
  • A microscope that uses electron source to magnify objects
  • Facem cunoștință cu laboratorul și cu ceilalți studenți
  • Prepararea culturilor lichide
  • Amplificarea ADN-ului
  • 50nm to 1mm
  • Preparation of liquid cultures
  • Παίρνετε τα αποτελέσματα των εργαστηριακών εξετάσεων και των ακτινογραφιών
  • Protocolul experimental pentru testul Fehling
  • They will buy a bookcase.
  • Results
  • 1cm to 10m
  • Preparación de cultivos líquidos
  • DNA Isolation: Experimental steps

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