Check the experimental set-up after 48hrs

Two days later, the students observe the plates under the microscope and notice that several wells show signs of unexpected stress, such as shriveled, sparse cells and cloudy media. Dr. Lee addresses the group to guide their troubleshooting process.

Dr. Lee: ‘Some of these wells are showing more stress than we expected based on our experimental conditions. Let’s systematically troubleshoot what might have gone wrong.’

What is the most likely explanation for the unexpected stress?