37oC, 1% O2 levels, 5% CO2 levels.

You: ‘What about this one? Hypoxic conditions could be interesting if we want to simulate a tumor microenvironment.’

Dr. Lee: ‘Great observation—hypoxia is indeed relevant to studying tumors. However, for this experiment, we’re examining general cell behavior under stress with treatment. Hypoxic conditions might introduce additional variables and make results harder to interpret. This would be better suited for follow-up experiments with a more defined focus.’

We still have two options to consider. Which is more likely?