Check the experimental set-up after 48hrs

Two days later, the students observe the plates under the microscope and notice that several wells show signs of unexpected stress, such as shriveled, sparse cells and cloudy media. Dr. Lee addresses the group to guide their troubleshooting process.

 

Dr. Lee: ‘Some of these wells are showing more stress than we expected based on our experimental conditions. Let’s systematically troubleshoot what might have gone wrong.’

 

What is the most likely explanation for the unexpected stress?

  • The plate was not incubated at the appropriate temperature or CO₂ level.
  • Contamination of media or poor aseptic technique.
  • Incorrect preparation of the nutrient-deprived media.

Map: CS14 - CELL CULTURE (1063)
Node: 20074
Score:

reset

OpenLabyrinth
OpenLabyrinth is an open source educational pathway system

Review your pathway

  • Σπάργωση ή σπαργή (σε υποτονικό διάλυμα) και αδράνεια (σε ισοτονικό διάλυμα)
  • You and Emma might have forgotten to tare the balance with the plastic bag in place.
  • Οι μεταβολές του pH μπορούν να οδηγήσουν στη μετουσίωση του αμύλου
  • If we want to wash our eyes because they have dried out
  • Το pH επηρεάζει τον ιονισμό του αμύλου
  • Το pH επηρεάζει τον ιονισμό της αμυλάσης
  • Western Blot
  • Quantification of bacterial load: Inoculating the bacterial samples on agar plates
  • The fertilizer was added while the window was open, allowing air currents to interfere.
  • There is a potential connection between the current case and the one from 1989
  • Focus only on glucose control
  • Cis-Platinum + Gemcitabine
  • Explain medications quickly without checking understanding
  • What to do next?
  • Image A is from TEM and image B is from SEM
  • There is no connection between the current case and the one from 1989
  • Check the experimental set-up after 48hrs

Reminder

empty_reminder_msg

FINISH

Time is up