37⁰C, atmospheric O2 levels, atmospheric CO2 levels.

Michael: ‘What about atmospheric oxygen and carbon dioxide levels?  Our cells, which are mammalian cells, function effectively in these conditions.’

Dr. Lee: “That’s true, Michael however in cell culture, these conditions are often tailored to bacteria. However, for mammalian cells, the absence of CO₂ would destabilize their environment, leading to poor pH regulation and stress. Mammalian cells like the ones in this experiment require different parameters to survive and function. Let’s refine our thinking to match our objective.’

We still have two options to consider. Which is more likely?