Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Microscopic Observation
  • Gel electrophoresis
  • Western Blot
  • Spectrophotometer

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA_ES (1021)
Node: 19394
Score:

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  • Mutarea într-o casă nouă.
  • Παθολογικές σχέσεις
  • Ενδοαυλική αποκατάσταση
  • Práctica de ósmosis en células animales
  • Learning Point
  • Results: Effect of pH on amylase activity
  • Está agresivo y no quiere presentarse.
  • Συμπεράσματα
  • Επανέλεγχος με ούρα 24ώρου μετά από 8-12 εβδομάδες και αν ο έλεγχος είναι φυσιολογικός μετά από 12 μήνες
  • ΝΕΦΡΙΚΗ ΑΝΕΠΑΡΚΕΙΑ
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  • ΘΡΟΜΒΩΣΗ ΑΓΓΕΙΩΝ ΑΣΘΕΝΟΥΣ
  • Efecto del pH en la actividad de la amilasa
  • Learning Point
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  • ΣΥΓΧΑΡΗΤΗΡΙΑ!!!
  • Yes
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  • Amplificarea ADN-ului
  • Chlorpropamide
  • Επιλογή 3
  • Opțiunea 2
  • Ligaza ADN
  • Plating your cells
  • Node 11: Discuss involving a family member for additional support
  • Game Over
  • To enhance the binding affinity of restriction endonucleases to non-specific sequences
  • Glomerular filtration rate
  • Methimazole, propranolol, hydrocortisone, iodine solution
  • Afterload
  • Contractility
  • Επιλογή 3
  • Epilogue
  • Propylthiouracil, propranolol
  • Setting up the reaction

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