Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Spectrophotometer
  • Western Blot
  • Gel electrophoresis
  • Microscopic Observation

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
Node: 19338
Score:

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  • Εσείς και η Έμμα μπορεί να ξεχάσατε να μηδενίσετε (tare) τον ζυγό με το πλαστικό σακουλάκι τοποθετημένο πάνω του.
  • Areas B and D are contaminated and have a high bacterial load.
  • To ensure proper folding and function of the restriction endonuclease, maximizing its catalytic activity
  • Your detail explanation
  • Isosorbide mononitrate
  • Areas B, C and D are contaminated and have a high bacterial load.
  • Digestia cu endonucleaze de restricție: Condiții optime
  • MSA
  • Answer 2
  • Πείραμα PCR
  • Oul de broască
  • To stabilize the DNA double helix structure, ensuring precise cleavage
  • To protect us from any spills. White is a colour where most spillages are visible depending solution colour
  • Thank you—that’s helpful. Let’s quickly check your strength now
  • Areas B, C and D are contaminated.
  • Afterload
  • Dopamine agonist
  • Vasopressin analogue
  • Tries to reduce her alcohol consumption
  • Bisoprolol
  • Περιγραφή 3
  • Iodine
  • It's not stroke
  • A
  • Spironolactone (aldosterone/mineralocorticoid receptor antagonist)
  • Node 7: Encourage Maria to practice using the system
  • Heart rate
  • Setting up the reaction

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