Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Spectrophotometer
  • Western Blot
  • Microscopic Observation
  • Gel electrophoresis

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
Node: 19338
Score:

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  • Areas B, C and D are contaminated.
  • Purple
  • (1) προφάση, (2) μεσοφάση, (3) τελόφαση, (4) προμεταφάση, (5) μετάφαση και (6) ανάφαση
  • "This is likely a coincidental acute viral hepatitis. Continue the anti tuberculosis drug to prevent resistance. Give a liver supplement and refer for hepatitis serology.
  • COPDAC (Cyclophosphamide, Vincristine/Oncovin, Prednisone, Dacarbazine)
  • White
  • Οχι, τον παραπέμπεις σε εξειδικευμένο κέντρο
  • 1
  • BEACOPP (Bleomycin, Etoposide, Adriamycin/Doxorubicin, Cyclophosphamide, Oncovin/Vincristine, Procarbazine, Prednisone)
  • Ionuț va cere bani de la mama sa.
  • 10/10000
  • Balanzas de laboratorio II
  • Option 3
  • Option 2
  • Game Over
  • Data ditemukan
  • Game Over
  • Learning Point
  • Προτείνεις να επισκεφτείς όλα τα σπίτια της περιοχής.
  • Setting up the reaction

Reminder

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FINISH

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