Setting up the reaction

Dr. Greene explains that there are many commercially available kits and the lab uses the one from Thermo Scientific.

https://www.thermofisher.com/order/catalog/product/FD0275 

 

You pipette each of the components into a clean Eppendorf tube in the order below. You also remember to add a negative control without DNA. You also run a DNA sample from the victim to investigate whether the DNA from the T-shirt is the victim’s.

 

Water, nuclease-free 

30µL

EcoRI buffer

5 µL

DNA

10 µL (5 µg)

EcoRI enzyme

5 µL

Total volume

50 µL

 

You incubate the reaction at 37oC for 30 minutes. The reaction is stopped through thermal inactivation at 80oC for 5 minutes. 

You are anxious to find out the results and understand that you need to do a bit more work to visualize the results. 

Which technique is appropriate to visualize the results?

  • Spectrophotometer
  • Microscopic Observation
  • Gel electrophoresis
  • Western Blot

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
Node: 19338
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  • El e fericit.
  • Norčuje se iz učiteljice Katje.
  • Dacă ți-au intrat în ochi substanțe chimice periculoase
  • Η γιαγιά φοράει μάσκα και γάντια.
  • Αποτελέσματα: επίδραση του pH στη δραστικότητα της αμυλάσης
  • Προστασία από τον ήλιο.
  • Παρατήρηση κυττάρων μετά από 12–18 ώρες επώασης
  • Delay wound assessment until an in-person visit
  • Απλή παρακολούθηση
  • Results: Effect of pH on amylase activity
  • Epilogue
  • The patient will be dissatisfied, and might not come to the next appointment and you will lose the follow up. You will also not have enough data about patient’s progress.
  • El e fericit.
  • Balanzas de laboratorio II
  • Small samples and inner structural information needed
  • Alineación, Desnaturalización, Extensión
  • A
  • Γράφεις όλες τις απαραίτητες πληροφορίες στην αφίσα: το μπαζάρ και τον σκοπό για τον οποίο γίνεται, την ημέρα, την ώρα και την τοποθεσία.
  • Game Over
  • Bisoprolol (beta blocker)
  • Larger area sample and when surface information is needed
  • Setting up the reaction

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