Restriction Endonuclease Digestion of DNA: Selecting the Right Enzyme

Having thoroughly reviewed your notes, you are confident that you understand the principles behind this technique. 

Dr. Greene: Well then, let’s get started. We'll need to perform restriction enzyme digestion to prepare the DNA fragments for further examination. Let's discuss which enzyme might be the best fit for this task.

Eric: Molecular biology really relies on a variety of enzymes. 

You: That’s right, we learnt about several molecular biology techniques, with many of them utilizing enzymes, which are important in research and diagnostics.

Dr. Greene: Exactly, and in a forensic context, enzymes are invaluable for DNA digestion. By cutting DNA at specific sequences, they allow us to generate fragment patterns unique to individuals, facilitating comparisons in investigations. 

 

Source: Freepik

Given the task at hand, what enzyme would be most appropriate?

  • EcoRI
  • Reverse transcriptase
  • Taq polymerase
  • DNA Ligase

Map: CS9 - RESTRICTION ENDONUCLEASE DIGESTION OF DNA (1018)
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